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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jan 14;1860(3):374–382. doi: 10.1016/j.bbagrm.2017.01.005

Fig. 5. Translational regulation of TK1 is mediated by Fhit scavenger decapping activity.

Fig. 5

A. Parental H1299 cells were co-transfected with plasmids expressing firefly luciferase with the TK1 5’-UTR and Renilla luciferase, and either empty vector or plasmids expressing WT Fhit or the catalytically inactive H96N mutant. The impact of Fhit catalytic activity on expression driven by the TK1 5’-UTR was determined by dual luciferase assays. Data shown are the means ±SEM of three independent experiments (each measured in triplicate). The asterisk (*) indicates p <0.05 as determined by two-tailed Student T test. B. Extracts from DcpS positive HCT116 cells and H1299 cells used in this study were analyzed by Western blotting for DcpS and for Vinculin. C. H1299 E1 cells were transfected with control or DcpS siRNA and monitored by Western blotting for DcpS, Fhit, Vinculin and TK1.