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Current protocol for IL6 assay with the immunoassay chip system. Time of each assay step is in bold.
| Parameters | |
|---|---|
| Flow rate | 30 μl/min |
| Primary Ab coating | 5 μg/ml 2 h |
| Sample time | 10 min |
| Secondary Antibody | 4 μg/ml for 10 min |
| Reporter | 0.5 μg/ml for 10 min |
| Washing | 25 min |
| Substrate | 6 min |
| Detection | 5 min |
| Total time | 66 min |
