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. 2016 Dec 21;37(2):133–142. doi: 10.1007/s10875-016-0363-5

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Frequency CD28null and CD57-expressing T cells. Frequencies of peripheral CD57⁺ cells within the CD4⁺ (a) and CD8⁺ (b) T cells were determined in both NBS patients (n = 20, black dots) and HI (n = 125, open dots). Next, the loss of co-stimulatory molecule CD28 within the effector fraction (CD45RO⁻CD197⁻) of the CD8⁻ (CD4) (c) and CD8⁺ (d) T cell population was determined. Both groups were divided on the basis of their age into four groups (age distribution (n = HI vs. NBS), respectively): 0–2 (n = 26 vs. 7), 2–5 (n = 27 vs. 3), 5–16 (n = 50 vs. 6), and 16+ years old (n = 22 vs. 3). Data represents individual measurements and medians. Significant differences between patients and HI and between different age groups were calculated and shown (* = p < 0.05, ** = p < 0.01, *** = p < 0.001)