Table 1.
Six-color antibody panel for analyzing the composition of the T cell compartment
| Tube | FITC | PE | PerCP | PE-Cy7 | APC | APC-H7 | |
|---|---|---|---|---|---|---|---|
| Tru Count | CD3 (SK7) A |
CD16/CD56 (B37.1)/(NCAM16.2) A |
CD45 (2D1) A |
CD4 (SK3) A |
CD19 (SJ25C1) A |
CD8 (SK1) A |
|
| 1. | TCRαβ (WT31) A |
TCRγδ (11F2) A |
CD3 (SK7) A |
CD4 (SK3) A |
CD8 (SK1) A |
αβ vs. γδ | |
| 2. | CD45RO (UCHL1) B |
CD45RA (2H4) C |
CD3 (SK7) A |
CD4 (SK3) A |
CD27 (L128) A |
CD8 (SK1) A |
Maturation |
| 3. | CD28 (CD28.2) A |
CD197 (3D12) A |
CD3 (SK7) A |
CD8 (SK1) A |
CD45RO (4CHL1) A |
CD27 (M-TL71) A |
Maturation |
| 4. | CD57 (HNK-1) A |
CD3 (SK7) A |
CD4 (SK3) A |
CD8 (SK1) A |
Effector | ||
| 5. | Vδ1 (R9.12) D |
Vδ2 (B6) A |
CD3 (SK7) A |
CD4 (SK3) A |
TCRαβ (IP-26) E |
CD8 (SK1) A |
γδ subset |
| 6. | Vδ2 (B6.1) A |
Vγ9 (B3.1) A |
CD3 (SK7) A |
CD4 (SK3) A |
TCRαβ (IP-26) E |
CD8 (SK1) A |
γδ subset |
Clones are shown between brackets, manufactures by characters: A—BD Biosciences, San Jose, CA, USA; B—Dako, Herverle, Belgium; C—Beckman Coulter, Woerden, the Netherlands; D—Immunotech, Marseille, France; E—eBioscience, San Diego, Ca, USA