Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 20;133(3):463–483. doi: 10.1007/s00401-016-1658-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 6 — Free-choice alcohol drinking reduces sphingomyelin levels in the dorsal hippocampus (DH) of mice over-expressing acid sphingomyelinase (tgASM). The MALDI images were acquired with x–y-raster widths of 30 µm for coronal sections. Slice MALDI mass spectrograms for the three most abundant sphingomyelin (SM) species in the neuropil of the DH of water or 16 vol% alcohol (EtOH) drinking tgASM or wild type (WT) mice with regular consumption and three withdrawal and reinstatement episodes. The DH was analyzed as a target area of interest. The errorbars show the mean of eight samples/genotype–treatment (±SEM) for four animals. The data are shown using a rainbow scale that is normalized against the total ion count. a The coronal tissue sections show that SM 18:1 18:0 is enriched in the hypothalamus, amygdala, and DH. b The abundance of SM 18:1 18:0 was not changed in tgASM mice (p > 0.05). Alcohol drinking reduces levels of SM 18:1 18:0 in WT and tgASM mice to the same degree (ANOVA, factor treatment: F _1,28 = 63.281, p < 0.001). c The coronal tissue sections show that SM 18:1 18:1 is particularly enriched in the amygdala and hypothalamus. d The abundance of SM 18:1 18:1 was not changed in tgASM mice (p > 0.05). Alcohol drinking reduces levels of SM 18:1 18:1 in WT and tgASM mice to the same degree (ANOVA, factor treatment: F _1,28 = 80.920, p < 0.001). e The coronal tissue sections show SM 18:1 20:0 enriched in the thalamus and ventricles. f The abundance of SM 18:1 20:0 was not changed in tgASM mice (p > 0.05). Alcohol drinking reduces levels of SM 18:1 20:0 in WT and tgASM mice to the same degree (ANOVA, factor treatment: F _1,28 = 36.964, p < 0.001). g Mass spectrogram for a single slice point at the DH level with SM target masses. h Anatomical analysis of the slice at level of the Nac (Amyg amygdala, cc corpus callosum, ci commisura interior, Ctx cortex, dHipp dorsal hippocampus, Hyp hypothalamus, Thal thalamus). For an overview on white matter and liquor associated SM species, see Suppl. Figure 8, 9 (***p < 0.001)