Table 1.
Some features of predicted miRNAs from EST analysis of C. sativus
| Known mature miRNA | ppt-miR414 | aly-miR837-5p |
|---|---|---|
| Input sequence name | Contig65 | Contig441 |
| Number of mismatches between miRNA and miRNA* | 3 | 4 |
| Number of bulges | 2 | 0 |
| Bulge size | 1 | 0 |
| Number of flanked bases | 2 | 8 |
| Precursor start | 174 | 251 |
| Precursor stop | 231 | 450 |
| Primary miRNA MFE(kcal/mol) | -187.405 | -293.481 |
| Precursor miRNA MFE(kcal/mol) | -28.295 | -66.852 |
| Precursor miRNA MFEI(kcal/mol) | -0.832 | -0.777 |
| Number of homolog mismatches | 4 | 4 |
| Known mature miRNA sequence | 5':UCAUCCUCAUCAUCCUCGUCC:3' | 5':CAUUGUUUCUUGUUUUUUUCA:3' |
| Predicted miRNA sequence | 5':UCCCUCUCAUCAUCCUCGUCG:3' | 5':CUUUGUUUCUUGUUUUCCUCC:3' |
The maximum asymmetry of miRNA/miRNA
*
duplex was 4 for prediction of a novel miRNA