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. 2016 Oct 14;8(3):202–218. doi: 10.1007/s13238-016-0324-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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UHRF2 regulates the expression of H3K9ac and H3K14ac. (A) HEK293, LO2 and HepG2 cells were transfected with the indicated plasmids. Cellular lysates were analyzed by Western blot. UHRF2 was detected using anti-Flag antibodies. H3 and GAPDH were used as loading controls. (B) H3K9ac and H3K14ac levels were normalized to 1 at control groups. Significance was represented as *P < 0.05. (C and D) Endogenous UHRF2 protein levels were decreased by shRNA against UHRF2. The cellular lysates were analyzed by Western blot and quantified using Image J software. (E) Various UHRF2 deletion mutants; (F–H) HEK293, LO2 and HepG2 cells were transiently transfected with indicated plasmids. After 48 h, the cellular lysates were analyzed by Western blot. (I and J) Immunofluorescence analyses were performed using anti-H3K9ac or anti-H3K14ac antibodies

UHRF2 regulates the expression of H3K9ac and H3K14ac. (A) HEK293, LO2 and HepG2 cells were transfected with the indicated plasmids. Cellular lysates were analyzed by Western blot. UHRF2 was detected using anti-Flag antibodies. H3 and GAPDH were used as loading controls. (B) H3K9ac and H3K14ac levels were normalized to 1 at control groups. Significance was represented as *P < 0.05. (C and D) Endogenous UHRF2 protein levels were decreased by shRNA against UHRF2. The cellular lysates were analyzed by Western blot and quantified using Image J software. (E) Various UHRF2 deletion mutants; (F–H) HEK293, LO2 and HepG2 cells were transiently transfected with indicated plasmids. After 48 h, the cellular lysates were analyzed by Western blot. (I and J) Immunofluorescence analyses were performed using anti-H3K9ac or anti-H3K14ac antibodies