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. 2016 Dec 1;8(3):191–201. doi: 10.1007/s13238-016-0345-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Anti-IL-25 antibody treatment dampened the polarization of M2 macrophages in primary tumor. The MMTV-PyMT mice were treated with either Rat IgG or anti-IL-25 antibody starting from 4 weeks old to 14 weeks old (3–7 mice/group), and then sacrificed at 14 weeks old for the following analysis. (A) Staining of CD206 positive macrophage populations in mammary carcinomas (shown as mean ± SE). (B) The relative mRNA level of YM-1 and Arg in sorted tumor macrophages or the relative mRNA level of Il10, Il12, Tgfb and Tnfa genes in the total tumor tissue