Table 1. Gradient flow HPLC conditions for filgrastim assay .

Time (Min)	Mobile phase A (%v/v)	Mobile phase B (%v/v)
0-4	92	8
4-19	92 to 72	8 to 28
19-19.1	72 to 0	28 to 100
19.1- 21	0	100
21- 21.1	0 to 92	100 to 8
21.1- 25	92	8