|
| |||
| Component | Amount per reaction (μl)
|
Final | |
| RNA sample | oGAB11 control | ||
| PEG8000 (50% vol/vol) | 8.0 | 8.0 | 20% (vol/vol) |
| DMSO | 2.0 | 2.0 | 10% (vol/vol) |
| T4 RNA Ligase Buffer (10×) | 2.0 | 2.0 | 1× |
| Barcode DNA Linker (1 μg) | 1.0 | 1.0 | |
| RNA sample (1 μg) | 6.0 | – | |
| oGAB11 (10 μM) | – | 1.0 | 0.5 μM |
| RNAse-free H2O | – | 5.0 | |
| Truncated T4 RNA Ligase 2 | 1.0 | 1.0 | 200 U |
|
| |||
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