|
| |||
| Component | Amount per reaction (μl) | Final | |
|
| |||
| CircLigase 10× Reaction Buffer | 2.0 | 1× | |
| ATP (1 mM) | 1.0 | 50 μM | |
| MnCl2 (50 mM) | 1.0 | 2.5 mM | |
|
| |||
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