|
| ||
| Component | Amount per reaction (μl) | Final |
|
| ||
| Circularization reaction (from Step 70) | 5.0 | |
| Depletion DNA oligo pool | 1.0 | 1 μM (per Depletion oligo) |
| 20× SSC | 1.0 | 2× |
| DNase-free H2O | 3.0 | |
|
| ||
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