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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Nat Protoc. 2016 Mar 24;11(4):813–833. doi: 10.1038/nprot.2016.047
Component Amount for 4.5 reactions (μl) Final
Phusion HF Buffer, 5× 18 1×
dNTPs (10 mM) 1.8 0.2 mM
Forward primer (Illumina Index primer, 100 μM) 0.45 0.5 μM
oNTI231 (Reverse primer, 100 μM) 0.45 0.5 μM
DNase-free H2O 63.9
Phusion DNA Polymerase (2 U/μl) 0.9 1.8 U