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Flumazenil can act as a competitive antagonist at high GABA concentrations. A, Representative traces of GABA-evoked chloride currents in the presence and absence of 4 μM flumazenil. Black traces are GABA alone, orange traces are co-application of GABA and flumazenil. B, Summative dose-response curves for application of GABA and GABA plus flumazenil to α1β2γ2s receptors (n = 8 cells). Error bars represent SEM.
