. Author manuscript; available in PMC: 2018 Jan 20.

Published in final edited form as: ACS Chem Biol. 2016 Dec 20;12(1):300–310. doi: 10.1021/acschembio.6b00954

Table 1.

Catalytic efficiencies of SIRT7 on H3 acyl peptides with different nucleic acids

Acyl peptide	activator	k_cat (min⁻¹)	K_m (µM)	k_cat/K_m (M⁻¹s⁻¹)
H3K18 Ac	-	NP	NP	NP
H3K18 Ac	tRNA	1.2 ± 0.2	309 ± 59	65

H3K18 Myr	-	NP	NP	NP
H3K18 Myr	tRNA	0.02 ± 0.002	<2	>167

H3K9 Ac	-	NP	NP	NP
	dsDNA	ND	ND	1.0
	tRNA	ND	ND	1.0

H3K9 Myr	-	0.004 ± 0.001	65 ±26	1.1
	dsDNA	0.04 ± 0.005	32 ±10	21
	tRNA	0.1 ± 0.01	41 ± 7	41

H3K9 But	tRNA	ND	ND	2.0
H3K9 Oct	tRNA	ND	ND	2.0

ND: Not determined because V~[S] was linear. Only k_cat/K_m value could be obtained.

NP: No product formed (product formation was below HPLC detection limit).

Ac: acetyl; But: butyryl; Oct: octanoyl; Myr: myristoyl.