. Author manuscript; available in PMC: 2018 Jan 20.

Published in final edited form as: ACS Chem Biol. 2016 Dec 20;12(1):300–310. doi: 10.1021/acschembio.6b00954

Table 3.

Catalytic efficiencies of SIRT7 truncates on H3K18 Ac and H3K9 Myr peptides

SIRT7	Acyl Peptide	activator	k_cat (min⁻¹)	K_m (µM)	k_cat/K_m (M⁻¹s⁻¹)	relative catalytic efficiency to FL
FL	H3K18 Ac	-	NP	NP	NP	NA
	H3K18 Ac	tRNA	1.2 ± 0.2	309 ± 59	65	1
	H3K9 Myr	-	0.004 ± 0.001	65 ±26	1.1	1
	H3K9 Myr	tRNA	0.1 ± 0.01	41 ± 7	41	1

ΔN	H3K18 Ac	-	NP	NP	NP	NA
	H3K18 Ac	tRNA	0.1 ± 0.003	294 ± 15	5.7	0.09
	H3K9 Myr	-	0.002 ± 0.0002	90 ± 16	0.4	0.36
	H3K9 Myr	tRNA	0.02 ± 0.002	18 ± 4.2	19	0.46

ΔC	H3K18 Ac	-	NP	NP	NP	NA
	H3K18 Ac	tRNA	NP	NP	NP	NA
	H3K9 Myr	-	0.003 ± 0.0003	18 ± 4.7	2.8	2.5
	H3K9 Myr	tRNA	0.04 ± 0.006	25 ± 8.6	27	0.66

core	H3K18 Ac	-	NP	NP	NP	NA
	H3K18 Ac	tRNA	NP	NP	NP	NA
	H3K9 Myr	-	0.001 ± 0.0001	89 ± 22	0.2	0.2
	H3K9 Myr	tRNA	0.005 ± 0.0003	39 ± 4.5	2.1	0.05

NP: No product formed (product formation was below HPLC detection limit).

NA: Not available.

FL: 1–400; ΔN: 56–400; ΔC: 1–364; core: 56–364.