GW2580 treated mice display ameliorated depression-like behavior and increased microglial activation. (A) Depression-like behavior was assessed in the Porsolt swim test for 3 minutes. (B) Representative images of IBA-1 staining in the cortex from the PBS and GW2580 treated groups is shown, along with the intensity of IBA-1 staining as measured by ImageJ. (C) Representative images of H&E staining in the third ventricles of PBS and GW2580 treated mice. (D) Representative images of albumin staining are shown, with arrows indicating the blood vessels. The number of animals in the forced swim test in the PBS and GW2580 treated groups is 10 and 10, respectively. For IBA-1, H&E, and albumin staining, the number is 10 and 9, respectively.