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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Connect Tissue Res. 2016 Jun 16;58(1):15–26. doi: 10.1080/03008207.2016.1200566

Figure 2. The inhibitory effect of ELF3 on Sox9-driven transcription does not depend upon binding of ELF3 to ETS binding sites.

Figure 2

The C-28/I2 cells were co-transfected with the pLuc 4×48 enhancer construct containing the 89-bp COL2A1 promoter with four 48-bp enhancer elements and with (A) expression vectors encoding ELF3 or (B) expression vectors encoding Sox9 and Sox6 alone or in combination with ELF3. (C) The C-28/I2 cells were co-transfected with the Gal TK-luc reporter construct and expression vectors encoding Gal4-Sox9 alone or together with ELF3. ***indicates p<0.001.