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. 2017 Feb 27;11:45. doi: 10.3389/fncel.2017.00045

Figure 1.

Figure 1

Demyelination of the main axon of thick-tufted layer 5 pyramidal neurons. (A,B) Overview fluorescent image of a myelinated (A) demyelinated (B) parasagittal brain section immunolabeled for myelin basic protein (MBP). Red asterisks, indicate the locations of the recorded thick-tufted layer 5 neuron shown in (C). Note that cuprizone-induced gray matter demyelination (yellow arrowheads) occurs across the entire cerebral cortex. S1HL, primary somatosensory hindlimb cortex; V2, secondary visual cortex; M1, primary motor cortex; M2, secondary motor cortex; CC, corpus collosum; Hip, hippocampus. Scale bar, 700 μm, (C) z-projected confocal images of layer 5 axons co-labeled for biocytin (red) and MBP expression (cyan). Yellow arrowheads, indicate branch points (BPs) along the main axon. Scale bar, 20 μm. (D) Magnified z-projected confocal images of BPs of the demyelinated primary axon shown in (C). Yellow arrowheads, BPs. Red arrowheads, trajectory of the primary and secondary axon collaterals. Scale bar, 5 μm.