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. 2017 Feb 27;11:10. doi: 10.3389/fnana.2017.00010

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Copyright © 2017 Marking, Krosnowski, Ogura and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative analysis of cholinergic nerve fiber density in various layers of the aAOB and pAOB. (A) A low-magnification image of the AOB taken from a sagittal section. VAChT-ir positive nerve fibers are present in the GL, MCL and GCL. (B) Image of the enlarged middle AOB region that has been processed using Hessian-based feature extraction for automatic fiber count (for detailed method, see Krosnowski et al., 2012 and Sathyanesan et al., 2012). (C) Plot of VAChT-ir fiber density (Mean ± SEM, n = 3 mice). A higher density of VAChT-ir positive fibers is found in MCL and GCL than in the GL, with the fiber density in the pMCL trending toward being the highest. Scale: (A) 200 μm (B) 50 μm.