Table 4.
Gliadin and glutenin contents (μg/mg flour) of low-gliadin transgenics and wild type lines for two different nitrogen treatments from Experiment 2.
| RP-HPLC |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gliadins |
Glutenins |
|||||||||
| Line | N (mg) | ω | α | γ | Total | HMW | LMW | Total | Prolamins | Gli/Glu |
| BW208 | 120 | 7.7 | 14.9 | 8.9 | 31.5 | 7.3 | 13.1 | 20.4 | 51.9 | 1.56 |
| 1080 | 21.7∗∗∗ | 42.6∗∗∗ | 28.8∗∗∗ | 93.1∗∗∗ | 19.4∗∗∗ | 28.1∗∗∗ | 47.5∗∗∗ | 140.6∗∗∗ | 1.96∗∗∗ | |
| D783 | 120 | 7.7 | 7.5 | 1.8 | 17.0 | 14.4 | 16.2 | 30.6 | 47.6 | 0.55 |
| 1080 | 11.1∗∗∗ | 15.6∗∗∗ | 5.4∗∗∗ | 32.1∗∗∗ | 32.3∗∗∗ | 33.6∗∗∗ | 65.9∗∗∗ | 98.0∗∗∗ | 0.49 | |
| D793 | 120 | 9.1 | 6.7 | 0.8 | 16.6 | 23.1 | 15.9 | 39.1 | 55.6 | 0.43 |
| 1080 | 11.1∗∗ | 8.3 | 1.3 | 20.7∗ | 32.8∗∗∗ | 19.7∗∗ | 52.5∗∗∗ | 73.3∗∗∗ | 0.39 | |
| E82 | 120 | 8.6 | 5.2 | 0.3 | 14.2 | 16.6 | 10.7 | 27.3 | 41.5 | 0.53 |
| 1080 | 9.7∗ | 5.6 | 0.4 | 15.7 | 20.0∗ | 11.0 | 31.1∗∗ | 46.8 | 0.51 | |
Gliadins and glutenins were determined by RP-HPLC. HMW, high molecular weight; LMW, low molecular weight.
Means are significantly different between treatments for each line and protein fraction, as determined by LSD multiple comparisons: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.