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. 2017 Feb 27;8:263. doi: 10.3389/fpls.2017.00263

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Copyright © 2017 Jacq, Pernot, Martinez, Domergue, Payré, Jamet, Burlat and Pacquit.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The secretory pathway is involved in both cell wall and plastids AtLTP2-TagRFP localization. The AtLTP2-TagRFP fusion protein was produced together with the endoplasmic reticulum (ER) marker sp-YFP-HDEL in N. benthamiana leaves which were subjected to Brefeldin A (BFA) treatment. The AtLTP2-TagRFP fluorescence signal is false-colored in magenta in (A,D,G). The bright-field (B) is displayed to visualize the cell morphology. ER marker (E) and chlorophyll auto-fluorescence (H) are false-colored in green and the merge is presented in white in the right column (C,F,I). Note that in presence of BFA, AtLTP2-TagRFP fluorescence is restricted to the ER-Golgi hybrid and BFA compartments. Scale bars: 40 μm.