FIGURE 5.

atltp2-1 etiolated seedlings show increased water-loss and toluidine blue (TB) permeability. (A) Water-loss kinetics of WT, atltp2-1 and complemented lines (5′2 and 14′3; expressed as % of fresh mass) were performed at 25°C. Points represent mean values ± SE (n = 3). Significant differences from WT were assessed by a Wilcoxon rank-sum test (^∗p-value ≤ 0.05). Dry mass was measured after 3 days at 25°C. No significant differences between the different lines were assessed using a Wilcoxon rank-sum test (p-value > 0.05) (B) Arabidopsis thaliana etiolated seedlings from 5-day-old WT and atltp2-1 were imaged along a 25 min kinetics following exit from the in vitro conditions. atltp2-1 hypocotyls displayed a gradually shrinkage whereas WT hypocotyls resisted better to the induced desiccation (n = 11). Hypocotyl diameters were measured using the ImageJ software. The results were analyzed by ANOVA and Tukey HSD test (α = 0.01) using R software. Different letters indicate significant differences at p-value < 0.01. (C) Permeability of etiolated hypocotyls was examined using the TB staining test. TB uptake was quantified spectrophotometrically at OD_{626 nm}. Results are presented as mean values ± SD (n = 5). Significant differences from WT were assessed by a Student’s t-test (^∗∗p-value < 0.01).