Fig. 3.

Bisulfite sequencing of the Runx2 promoter in mouse organs. Each row of circles indicates whether CpG sites are methylated (black) or unmethylated (white) in each read from −3,371 to −3,148 (left), −2,985 to −2,649 (middle) and from −2,505 to −2,039 bp (right) relative to the transcription start site (TSS). Three divided ranges cover all CpG sites from −3,371 to −2,039 bp. A gradient of methylation rates is detectable along the genomic sequence. CpG sites in the cerebrum and heart are methylated more frequently in the intermediate region than those in testes and bone. Asterisks and an arrowhead that marks CpG-2,505 are shown at statistically significantly dependent CpG sites during methylation (P<0.05 and P<0.01, respectively). A horizontal line at the bottom of the figure indicates the defined Runx2-DMR extending from −2,658 to −2,226 bp.