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Two dimensional-polyacrilamide gel electrophoresis (2D-PAGE) analysis of the effect of different nitrating agents on human recombinant MnSOD (hrMnSOD). hrMnSOD was exposed to nitrite and hydrogen peroxide in the presence of horseradish peroxidase (HRP) or peroxynitrite (ONOO−). Changes in isoelectric point are indicated with arrows. Reproduced and adapted from Demicheli et al. (30).
