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. 2017 Mar 1;26(7):280–298. doi: 10.1089/ars.2016.6686

Table 2.

List of Identified Polyubiquitinated Proteins by Mass Spectrometry

Spot SSP Uni Prot N° N° peptides MW Protein DS vs. Ctr Ya DS/AD vs. Ctr Oa DS/AD vs. DSa Ctr O vs. Ctr Ya
1 3408 P12277 3 42.64 Creatine kinase B-type 2.11 2.28    
    Q99961/2 2 41.49 Endophilin-A2 and -A1 2.11 2.28    
2 7511 P25705 3 59.75 ATP synthase subunit alpha, mitochondrial 1.67      
3 3202 P32119 6 21.88 Peroxiredoxin-2 2.59 3.45 2.04  
4 3203 D6R956 2 26.84 Ubiquitin carboxyl-terminal hydrolase L1 2.75 3.8 2.05  
5 6303 P04083 2 38.71 Annexin A1 2.96      
6 4613 Q16555 3 62.29 Dihydropyrimidinase-related protein 2 27.34     4.5
    P25705 3 59.75 ATP synthase subunit alpha 27.34     4.5
    P21281 2 56.5 V-type proton ATPase subunit B, brain isoform 27.34     4.5
7 3605 P10809 7 61.05 60 kDa heat shock protein 7.22      
8 4811 Q16891 15 82.62 Mitochondrial inner membrane protein 2      
    P06396 8 85.69 Gelsolin 2      
    P46459 9 82.59 Vesicle-fusing ATPase 2      
    A2A274 7 87.82 Aconitate hydratase 2      
9 2402 P60709/P63261 2 41.73 Actin cytoplasmic 1 and 2       4.5
10 5202 P30041 2 25.03 Peroxiredoxin-6       25.03
11 2603 Q13509 3 50.43 Tubulin beta-3 chain and -6 chain     0.32  
    P04350 2 49.58 Tubulin beta-4A     0.32  
    P30153 3 65.3 Serine/threonine protein phosphatase 2A     0.32  
    Q9UMX0 2 62.51 Ubiquilin-1     0.32  
12 3409 Q13885 3 49.9 Tubulin beta-2A   0.17 0.22  
    P31930 3 52.64 Cytochrome b-c1 complex subunit 1   0.17 0.22  
    Q16352 4 55.39 Alpha-internexin   0.17 0.22  
    Q9Y2T3 2 52.83 Guanine deaminase   0.17 0.22  
    F5H5D3 3 57.73 Tubulin alpha-1B chain   0.17 0.22  
13 4505 P30101 4 56.78 Protein disulfide isomerase A3     5.12  
    P21281 3 56.5 V-type proton ATPase subunit B, brain isoform     5.12  
14 5608 P55809 2 56.16 Succinyl-CoA:3- transferase 1     0.48  
    O43175 3 56.65 D-3-phosphoglycerate dehydrogenase     0.48  
15 1207 B7Z596 4 31.75 Tropomyosin alpha-1 chain   2.13    
    P06753 2 28.95 Tropomyosin alpha-3 chain   2.13    
    P61981 5 28.3 14-3-3 protein gamma   2.13    
16 6602 P04075 14 45.26 Fructose bisphosphate aldolase A   3.06    
    P09972 11 39.45 Fructose bisphosphate aldolase C   3.06    
    P24752 6 45.20 Acetyl-CoA acetyltransferase, mitochondrial   3.06    
    P62333 7 44.17 26S protease regulatory subunit 10B   3.06    
a

The fold of increase/decrease of polyubiquitylation of a specific protein, in the groups of analysis, is indicated in the last four columns.

ATP, adenosine triphosphate; Ctr O, control old; Ctr Y, control young; DS/AD, Down syndrome with Alzheimer's disease; MW, molecular weight; SSP, standard spot.