Table 2.
Spot | SSP | Uni Prot N° | N° peptides | MW | Protein | DS vs. Ctr Ya | DS/AD vs. Ctr Oa | DS/AD vs. DSa | Ctr O vs. Ctr Ya |
---|---|---|---|---|---|---|---|---|---|
1 | 3408 | P12277 | 3 | 42.64 | Creatine kinase B-type | 2.11 | 2.28 | ||
Q99961/2 | 2 | 41.49 | Endophilin-A2 and -A1 | 2.11 | 2.28 | ||||
2 | 7511 | P25705 | 3 | 59.75 | ATP synthase subunit alpha, mitochondrial | 1.67 | |||
3 | 3202 | P32119 | 6 | 21.88 | Peroxiredoxin-2 | 2.59 | 3.45 | 2.04 | |
4 | 3203 | D6R956 | 2 | 26.84 | Ubiquitin carboxyl-terminal hydrolase L1 | 2.75 | 3.8 | 2.05 | |
5 | 6303 | P04083 | 2 | 38.71 | Annexin A1 | 2.96 | |||
6 | 4613 | Q16555 | 3 | 62.29 | Dihydropyrimidinase-related protein 2 | 27.34 | 4.5 | ||
P25705 | 3 | 59.75 | ATP synthase subunit alpha | 27.34 | 4.5 | ||||
P21281 | 2 | 56.5 | V-type proton ATPase subunit B, brain isoform | 27.34 | 4.5 | ||||
7 | 3605 | P10809 | 7 | 61.05 | 60 kDa heat shock protein | 7.22 | |||
8 | 4811 | Q16891 | 15 | 82.62 | Mitochondrial inner membrane protein | 2 | |||
P06396 | 8 | 85.69 | Gelsolin | 2 | |||||
P46459 | 9 | 82.59 | Vesicle-fusing ATPase | 2 | |||||
A2A274 | 7 | 87.82 | Aconitate hydratase | 2 | |||||
9 | 2402 | P60709/P63261 | 2 | 41.73 | Actin cytoplasmic 1 and 2 | 4.5 | |||
10 | 5202 | P30041 | 2 | 25.03 | Peroxiredoxin-6 | 25.03 | |||
11 | 2603 | Q13509 | 3 | 50.43 | Tubulin beta-3 chain and -6 chain | 0.32 | |||
P04350 | 2 | 49.58 | Tubulin beta-4A | 0.32 | |||||
P30153 | 3 | 65.3 | Serine/threonine protein phosphatase 2A | 0.32 | |||||
Q9UMX0 | 2 | 62.51 | Ubiquilin-1 | 0.32 | |||||
12 | 3409 | Q13885 | 3 | 49.9 | Tubulin beta-2A | 0.17 | 0.22 | ||
P31930 | 3 | 52.64 | Cytochrome b-c1 complex subunit 1 | 0.17 | 0.22 | ||||
Q16352 | 4 | 55.39 | Alpha-internexin | 0.17 | 0.22 | ||||
Q9Y2T3 | 2 | 52.83 | Guanine deaminase | 0.17 | 0.22 | ||||
F5H5D3 | 3 | 57.73 | Tubulin alpha-1B chain | 0.17 | 0.22 | ||||
13 | 4505 | P30101 | 4 | 56.78 | Protein disulfide isomerase A3 | 5.12 | |||
P21281 | 3 | 56.5 | V-type proton ATPase subunit B, brain isoform | 5.12 | |||||
14 | 5608 | P55809 | 2 | 56.16 | Succinyl-CoA:3- transferase 1 | 0.48 | |||
O43175 | 3 | 56.65 | D-3-phosphoglycerate dehydrogenase | 0.48 | |||||
15 | 1207 | B7Z596 | 4 | 31.75 | Tropomyosin alpha-1 chain | 2.13 | |||
P06753 | 2 | 28.95 | Tropomyosin alpha-3 chain | 2.13 | |||||
P61981 | 5 | 28.3 | 14-3-3 protein gamma | 2.13 | |||||
16 | 6602 | P04075 | 14 | 45.26 | Fructose bisphosphate aldolase A | 3.06 | |||
P09972 | 11 | 39.45 | Fructose bisphosphate aldolase C | 3.06 | |||||
P24752 | 6 | 45.20 | Acetyl-CoA acetyltransferase, mitochondrial | 3.06 | |||||
P62333 | 7 | 44.17 | 26S protease regulatory subunit 10B | 3.06 |
The fold of increase/decrease of polyubiquitylation of a specific protein, in the groups of analysis, is indicated in the last four columns.
ATP, adenosine triphosphate; Ctr O, control old; Ctr Y, control young; DS/AD, Down syndrome with Alzheimer's disease; MW, molecular weight; SSP, standard spot.