Fig 1.

Study design. Behavioral and MRI studies were performed in Nijmegen, the Netherlands. After a transient occlusion of the middle cerebral artery (tMCAo) for 30 min, mice were divided into two dietary groups (Control or Fortasyn). At 7 and 14 days post tMCAo all mice underwent MRI. In between, all mice were tested on motor and cognitive impairments via several behavioral tests, like the Open field, Rotarod, Pole test, Prepulse inhibition (Ppi), grip strength test, and novel object recognition test (ORT). After MRI, all brains were processed for immunohistochemical stainings. PET imaging studies were conducted in parallel in Münster, Germany. [¹⁸F]DPA-714 PET was conducted 7, 14 and 35 d after tMCAo in two dietary groups (Control or Fortasyn). T2w MRI for anatomical localization of stroke was conducted 14 d post tMCAo. The group sizes at the start and at the end of the experiments performed in Nijmegen, the Netherlands, and in Münster, Germany, are given. The group sizes shown in brackets indicates the number of mice used for the behavioral or imaging procedures and quantification. The dashed line indicates the period of time of exclusion of mice due to i.e. surgical problems, humane endpoint.