Skip to main content
. 2017 Jan 25;8(2):305–313. doi: 10.7150/jca.15753

Figure 2.

Figure 2

Luciferase analysis suggested MAP3K5 is a target gene of miR-BART22. A. Luciferase analysis of the interaction between miR-BART22 and its target sequences in the 3'-UTR of MAP3K5 mRNA in EBV-miR-BART22-5-8F, EBV-miR-BART22-5-8F added with miR-BAR22-mimics and that added with miR-BART22-inhibitors. B. Luciferase analysis in 5-8F, 5-8F added with miR-BAR22-mimics and that added with miR-BART22-inhibitors. The Renilla luciferase activity is expressed as relative luminescence units (RLU) normalized with a firefly luciferase construct. Values are shown as means ±standard deviation. C. EBV-miR-BART22 mimics transiently transfected into 5-8F, MAP3K5 mRNA and protein was detected. D. EBV-miR-BART22 inhibitor transiently transfected into C666-1, the level of MAP3K5 mRNA and protein was detected.