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. 2017 Feb 27;7:43587. doi: 10.1038/srep43587

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EMSA analysis showing the direct interaction of CcpA (a and b), GlnR (c and d), with PpflB or PpflA, respectively. Each lane contains approximately 30 ng PpflB or PpflA. CcpA and GlnR were used between 0.1 to 0.5 μM. The coding sequence of gyrB (30 ng) was used as a negative control. Gels were stained with SYBR Green EMSA for visualizing DNA.