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. 2017 Feb 27;7:43587. doi: 10.1038/srep43587

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sodium formate enhanced CcpA- and decreased GlnR affinity for PpflB (b and d, respectively) compared to CcpA (a), and GlnR (c) interaction without sodium formate. Each lane contains approximately 30 ng PpflB; 0.2–1.5 μM of CcpA, or GlnR with or without 10 mM sodium formate. The experiment was repeated three times, and a representative image is shown.