Table 1. Microarray analysis of gene expression in ∆pflB relative to wild type D39 grown anaerobically in CDM supplemented with galactose.
| GeneID | Function | Ratioa |
|---|---|---|
| SPD_0065 | Beta-galactosidase 3 | 3.2 |
| SPD_0066 | PTS system, IIB component | 2.6 |
| SPD_0067 | PTS system, IIC component | 2.4 |
| SPD_0068 | PTS system, IID component | 2.7 |
| SPD_0069 | PTS system, IIA component | 1.9 |
| SPD_0094 | Hypothetical protein | 2.3 |
| SPD_0095 | Hypothetical protein | 2 |
| SPD_0128 | MutT/nudix family protein | 2.3 |
| SPD_0145 | Hypothetical protein | 2 |
| SPD_0148 | Ransporter, major facilitator family protein | 2.8 |
| SPD_0149 | Hypothetical protein | 2.2 |
| SPD_0308 | ATP-dependent Clp protease, ATP-binding subunit, putative | 2 |
| SPD_0423 | ROK family protein | 2 |
| SPD_0447 | Transcriptional regulator, MerR family | 2.4 |
| SPD_0448 | Glutamine synthetase, type I glnA | 2 |
| SPD_0459 | Heat shock protein GrpE | 3 |
| SPD_0687 | ABC transporter, ATP-binding protein | 2.9 |
| SPD_0688 | Hypothetical protein | 2.9 |
| SPD_0915 | Iron-compound ABC transporter, iron compound-binding protein | 2 |
| SPD_0918 | Iron-compound ABC transporter, ATP-binding protein | 2 |
| SPD_1375 | Hypothetical protein | 2.3 |
| SPD_1524 | Transcriptional regulator, GntR family | 4.1 |
| SPD_1525 | ABC transporter, ATP-binding protein | 2.2 |
| SPD_1588 | Hypothetical protein | 2.1 |
| SPD_1589 | Hypothetical protein | 3 |
| SPD_1590 | General stress protein 24, putative | 2.6 |
| SPD_1591 | Hypothetical protein | 2.2 |
| SPD_1594 | Transcriptional regulator | 2.7 |
| SPD_1595 | Hypothetical protein | 2.7 |
| SPD_1644 | Hypothetical protein | 2.1 |
| SPD_1744 | Hypothetical protein | 2.5 |
| SPD_1745 | Transcriptional regulator PlcR, putative | 7.1 |
| SPD_1746 | Hypothetical protein | 13 |
| SPD_1749 | Bacteriocin formation protein, putative | 9.4 |
| SPD_1750 | Hypothetical protein | 11.5 |
| SPD_1751 | Hypothetical protein | 8.1 |
| SPD_1752 | Toxin secretion ABC transporter, ATP-binding/permease protein | 10.2 |
| SPD_1754 | Hypothetical protein | 5.5 |
| SPD_1755 | Hypothetical protein | 5.4 |
| SPD_1756 | ABC transporter, ATP-binding protein | 3.8 |
| SPD_1756 | Hypothetical protein | 2.5 |
| SPD_1830 | Glycosyl hydrolase, family 1 | 2 |
| SPD_1831 | PTS system, IIC component | 2.8 |
| SPD_1832 | PTS system, IIB component | 2.2 |
| SPD_1943 | Hypothetical protein | 2.1 |
| SPD_1944 | Secreted 45 kd protein | 4 |
| SPD_1945 | Rod shpae-determining protein MreD, putative | 4.6 |
| SPD_1946 | Rod shape-determining protein MreC | 4.9 |
| SPD_1947 | Hypothetical protein | 5.5 |
| SPD_1948 | ABC transporter, ATP-binding protein | 3.7 |
| SPD_1962 | Hypothetical protein | 11.4 |
| SPD_0116 | Hypothetical protein | −3.9 |
| SPD_0161 | Hypothetical protein | −2.6 |
| SPD_0262 | PTS system, mannose/fructose/sorbose family protein, IID component | −2.3 |
| SPD_0263 | manM PTS system, mannose-specific IIC component | −2 |
| SPD_0264 | manL PTS system, mannose-specific IIAB components | −1.7 |
| SPD_0378 | enoyl-CoA hydratase | −2.7 |
| SPD_0379 | Transcriptional regulator, MarR family | −2.3 |
| SPD_0380 | 3-oxoacyl-(acyl carrier protein) synthase | −2.4 |
| SPD_0381 | Acyl carrier protein | −2.5 |
| SPD_0382 | enoyl-(acyl-carrier-protein) reductase | −3.3 |
| SPD_0383 | Acyl-carrier-protein S-malonyltransferase | −3.6 |
| SPD_0384 | 3-ketoacyl-(acyl-carrier-protein) reductase | −4.3 |
| SPD_0385 | 3-oxoacyl-(acyl carrier protein) synthase | −5.2 |
| SPD_0386 | Acetyl-CoA carboxylase | −4.3 |
| SPD_0387 | (3 R)-hydroxymyristoyl ACP dehydratase | −4.6 |
| SPD_0388 | Acetyl-CoA carboxylase | −6.3 |
| SPD_0389 | Acetyl-CoA carboxylase beta subunit | −5.4 |
| SPD_0390 | Acetyl-CoA carboxylase alpha subunit | −5.6 |
| SPD_0407 | Hypothetical protein | −2 |
| SPD_0420 | Formate acetyltransferase | −2.9 |
| SPD_0471 | Pseudo | −2 |
| SPD_0511 | 5,10-methylenetetrahydrofolate reductase | −2.4 |
| SPD_0652 | Branched-chain amino acid ABC transporter, amino acid-binding protein | −1.6 |
| SPD_0653 | Branched-chain amino acid ABC transporter, permease protein | −1.9 |
| SPD_0654 | Branched-chain amino acid ABC transporter, permease protein | −2.3 |
| SPD_0655 | Branched-chain amino acid ABC transporter, ATP-binding protein | −2.7 |
| SPD_0656 | Branched-chain amino acid ABC transporter, ATP-binding protein | −2.5 |
| SPD_0727 | Hypothetical protein | −2.8 |
| SPD_0728 | Hypothetical protein | −2.2 |
| SPD_0729 | hemolysin-related protein | −2 |
| SPD_0730 | deoD purine nucleoside phosphorylase | −2.5 |
| SPD_0751 | Hypothetical protein | −2.3 |
| SPD_0752 | Hypothetical protein | −2.9 |
| SPD_0753 | pcp pyrrolidone-carboxylate peptidase | −2.9 |
| SPD_0761 | Hypothetical protein | −2.1 |
| SPD_0997 | hup DNA-binding protein HU | −2.1 |
| SPD_1016 | Serine/threonine protein phosphatase | −2 |
| SPD_1101 | Signal recognition particle-docking protein FtsY | −1.9 |
| SPD_1102 | Cof family protein | −2 |
| SPD_1103 | Cof family protein | −1.7 |
| SPD_1301 | NADPH-dependent FMN reductase | −2.2 |
| SPD_1302 | Oxidoreductase, putative | −2.2 |
| SPD_1360 | Hypothetical protein | −2.1 |
| SPD_1514 | ABC transporter, ATP-binding protein | −2 |
| SPD_1515 | Hypothetical protein | −2.1 |
| SPD_1632 | Hypothetical protein | −3.3 |
| SPD_1633 | galT−2 galactose-1-phosphate uridylyltransferase | −2.9 |
| SPD_1634 | galK galactokinase | −3.4 |
| SPD_1635 | galR galactose operon repressor | −1.9 |
| SPD_1636 | Alcohol dehydrogenase, zinc-containing | −2.8 |
| SPD_1637 | Transcriptional regulator, MerR family protein | −3.2 |
| SPD_1638 | Cation efflux system protein | −3.4 |
| SPD_1667 | Oligopeptide ABC transporter, ATP-binding protein AmiF | −2.4 |
| SPD_1668 | Oligopeptide ABC transporter, ATP-binding protein AmiE | −2.3 |
| SPD_1669 | Oligopeptide ABC transporter, permease protein AmiD | −2 |
| SPD_1670 | Oligopeptide ABC transporter, permease protein AmiC | −1.6 |
| SPD_1671 | Oligopeptide ABC transporter, oligopeptide-binding protein AmiA | −1.5 |
| SPD_1707 | Hypothetical protein | −2.5 |
| SPD_1797 | ccpA catabolite control protein A | −2.1 |
| SPD_1965 | Choline binding protein PcpA | −5.8 |
| SPD_2021 | Glycerol uptake facilitator protein | −2.1 |
| SPD_2033 | Ribosomal subunit interface protein | −2.3 |
aRatios ≥2 or ≤−2.0 (∆pflB compared with D39 wild type).
All P-values are <0.001.