Table 4. Expression levels (in Miller Units) of pneumococcal transcriptional lacZ-fusions to the promoters of ccpA and glnR in different backgrounds grown anaerobically in CDM supplemented with 55 mM of glucose or galactosea.
Strains | Glucose | Galactose |
---|---|---|
pPP1::lacZ-wt | 0.7 ± 0.05 | 0.9 ± 0.08 |
PccpA::lacZ-wt | 102.0 ± 1.5 | 451.2 ± 7.3 |
PccpA::lacZ-ΔccpA | 40.2 ± 3.0 | 14.7 ± 2.2 |
PccpA::lacZ-ΔglnR | 9.9 ± 0.2 | 324.1 ± 10.2 |
PglnR::lacZ-wt | 93.4 ± 1.8 | 565.0 ± 7.1 |
PglnR::lacZ-ΔccpA | 53.7 ± 2.5 | 147.8 ± 13.7 |
PglnR::lacZ-ΔglnR | 89.7 ± 4.4 | 572.3 ± 4.2 |
aThe activity is expressed as nmol p-nitrophenol/min/ml. Values are average of at least three independent experiments each with three replicates. ‘±’ indicates standard error of means (SEM).