Figure 4. Effect of SINV infection and sodium arsenite treatment on protein synthesis and eIF2α phosphorylation in HAP1 cell lines.
Mock-infected or SINV- infected cells from each HAP1 line were treated or not with 200 μM arsenite at 7 hpi during 1 h and 15 min and labeled with 35S-Met/Cys during the last hour of treatment. (A) Cells were collected in loading buffer and analyzed by SDS-PAGE and autoradiography to detect the protein synthesis. (B) Densitometric analysis of cellular and viral proteins synthesized in the absence or presence of arsenite in the different cell lines. The graph shows the percentage values obtained from untreated versus their counterpart cells treated with arsenite. The results are displayed as mean ± SD of three representative experiments. (C) The amount of phospho-eIF2α and total eIF2α was analyzed in parallel by western blotting. (D) As a control for the presence of eIF2A and eIF2D, the amount of these proteins was also analyzed by western blotting using a mixture of rabbit polyclonal anti-eIF2A and rabbit polyclonal anti-eIF2D as primary antibodies.