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. 2017 Feb 27;7:43876. doi: 10.1038/srep43876

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Copyright © 2017, The Author(s)

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(A) HAP1 WT, HAP1-eIF2A⁻ and HAP1-eIF2D⁻ cells were transfected with in vitro synthesized replicons rep C + luc, rep C + luc (ΔDLP), rep C + luc (Met-Leu) or rep C + luc (Met-Leu-ΔDLP). The DLP structure and the mutations introduced to generate the variant ΔDLP are indicated in Fig. 7B. At 7 hpt, cells were collected in loading buffer and analyzed by western blotting with an anti-C antibody. The mobilities of the C products derived from the different replicons are indicated by arrows. (B) Densitometric analysis of the different C proteins synthesized by the replicons in relation to the canonical product of C synthesized by rep C + luc in each cell line. The results are displayed as mean ± SD of three representative experiments.