Figure 3. Prolonged exposure to hydrogen peroxide does not affect cell proliferation or cell cycle progression.

(A) Plating efficiency from colony formation assays in untreated (CTRL) and H₂O₂-treated cells. The data are reported as mean ± s.e.m. (B) Quantification of live (solid lines) and dead (dashed lines) cells from flow cytometric analysis (volumetric absolute counting) performed over a four-day period (96 hours). Data are reported as mean ± s.d. (C) Cumulative population doubling level (CPDL) in untreated (CTRL) and H₂O₂-treated cells over a 21-day period. Data are reported as mean ± s.d. (D) Frequencies of β-galactosidase (senescence marker)-positive cells in untreated (CTRL) and H₂O₂-treated cells. Data are reported as mean ± s.e.m. (E) Data from FACS analysis showing proportion of cells in different phases of the cell cycle in untreated (CTRL) and H₂O₂-treated samples. Data are reported as mean ± s.d.