Figure 5. CD177^neg and CD177^mix neutrophils show similar migration in vitro and in vivo.

(a–c) 1.5 × 10⁶ sorted (CD177^mix and CD177^neg) or unsorted (CD177^presort) neutrophils were added to the upper well and migration towards buffer control (bu) or 10⁻⁸ M fMLF was assessed. Transmigrated neutrophils (a) through fibronectin (n = 4), (b) across HUVEC monolayer (n = 4), and (c) across glomerular MVEC monolayer in presence of control hu-IgG and PR3-ANCA (upper panel, n = 3) or isotype control and monoclonal anti-PR3 abs (lower panel, n = 3) are shown. ** is p < 0.01. (d) Neutrophils of single donors were isolated from the oral cavity (oral) and from blood in parallel (n = 6). The percentage of cells that were either positive for mPR3 or mCD177 was assessed by flow cytometry and was similar in neutrophils from both sites.