Figure 2. Relative level of glycosaminoglycan synthesis in HDFa, MPS IIIA, IIIB and VI fibroblasts after 72 h or 7-day treatment with different concentrations of acetaminophen, indomethacin, nimesulide, genistein (100 μM), mix 1 (10 μM indomethacin + 100 μM genistein), and mix 2 (25 μM nimesulide + 100 μM genistein), measured by incorporation of [³⁵S]O₄²⁻ or [³H]GlcN.

Radioactivity of incorporated precursors was quantified in a scintillation counter, calculated per DNA amount [dpm/ng DNA], and expressed as the percentage of control (‘Ctrl’ stands for cell culture treated with 0.05% DMSO). Statistical analysis was performed by using ANOVA with Tukey’s HSD Post Hoc test. Significance values are indicated with * for p < 0.05, ** for p < 0.01.