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. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: J Phys Chem B. 2017 Jan 25;121(5):1010–1019. doi: 10.1021/acs.jpcb.7b00088

Figure 2. TRIR difference spectra of AsLOV2 between 1 ps and 100 μs.

Figure 2

A. Upper panel, the experimental TRIR spectra for FMN in buffer at 2 ps (black) and 50 ns (red) compared (lower panel) with key stages in the AsLOV2 photocycle (2 ps; red, 50 ns; blue, 100 μs; green). 2B. The 100 μs TRIR spectrum of AsLOV2 compared with the steady state FTIR difference spectrum recorded after continuous irradiation. C. A 2D a red-blue ‘heatmap’ representation of the complete TRIR data showing three distinct regions ascribed to singlet, triplet and adduct. Yellow – red indicates a transient absorption, blue a bleach.