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. 2017 Feb 27;7:37569. doi: 10.1038/srep37569

Figure 3. 3DISCO analysis of DCC receptor expression in chicken, pigeon and duck embryos.

Figure 3

DCC (a) and ßIII-Tubulin (b) whole-mount immunostaining on HH22 chicken embryos after 3DISCO clearing. No DCC expression is detected in chicken embryos whereas many axonal tracts are strongly labeled with anti-ßIII-Tubulin. (c–j), By contrast, DCC is strongly expressed in pigeon (c,e–g) and duck (d,h–j) embryos at stages equivalent to HH22 or HH28. (h–j) in pigeon embryo, DCC is found in commissural axons crossing the floor plate (arrowhead in e), in retinal ganglion cells (Rgc) of the retina and the optic nerve (On; f), in the habenula nucleus (Ha) and fasciculus retroflexus (Fr; g). (h–j) in duck embryo, DCC is found in spinal cord (Sc) commissural axons, motor nerve roots (Mr), Rgc in the eye (i), optic nerve (On) diencephalon (Di) and olfactory nerve (Olf). Scale bars are 200 μm except (e,j), 100 μm and f, 50 μm. Abbreviations, Di: diencephalon; Hb: hindbrain; Tec, tectum; Olf: olfactory nerve; Tel, telencephalon; Tg, trigeminal ganglion.