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. 2017 Feb 27;7:43207. doi: 10.1038/srep43207

Figure 4. Analysis of the early secretory pathway in TRAPPC12−/− cells.

Figure 4

(A) Wildtype and TRAPPC12−/− HeLa cells were investigated with indicated markers of the early secretory pathway. (B) Wildtype and TRAPPC12−/− HeLa cells were stained with ERGIC53 after the cells were treated with or without autophagy inhibitor MHY 1485 at 10 μM for 2 hours. (C) Lysates of wildtype and TRAPPC12−/− HeLa cells were immunoblotted to detect the indicated proteins. (D) Wildtype and TRAPPC12−/− HeLa cells were depleted with siRNA specific to Firefly luciferase (control) or TRAPPC8 sequence and then stained with antibody against ERGIC-53. Scale bars = 10 μm.