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. 2017 Feb 27;7:43207. doi: 10.1038/srep43207

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Copyright © 2017, The Author(s)

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ER-to-Golgi transport was monitored by the transfected GFP-FM4-CD8 as transport marker. GFP-FM4-CD8 accumulated in the ER lumen in aggregated form until D/D solubilizer was applied to the cells for the indicated time. ER-to-Golgi traffic was monitored by co-staining with ERES marker Sec31A (A,C), or with Golgi marker GM130 (B,D). Scale bars = 10 μm.