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. 2017 Feb 27;7:43390. doi: 10.1038/srep43390

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(a) and (b) 3D reconstruction of the H1299 cells invading into the collagen based extracellular matrix for 24 hours. (c–e) 2D microscopic pictures of H1299 cell clusters just after the formation of 3D collagen extracellular matrix at the initial diameter of 200 μm (c), 400 μm (d) and 800 μm (e), respectively. (f–h) 2D microscopic pictures of invaded H1299 cells 24 hours in the 3D collagen extracellular matrix at the initial diameter of 200 μm (f), 400 μm (g) and 800 μm (h), respectively, with quantified equivalent invasion distances shown in (i) (*p < 0.05).