Figure 2. Killing of the mmpL3 conditional mutant in the presence of ATc.

The mutant was grown in roller bottles in Middlebrook 7H9 containing 500 ng/ml ATc. When the culture reached mid-log phase, bacteria were diluted in fresh media containing ATc (T0). Samples were collected at different time points and plated on Middlebrook 7H10 to determine the number of colony forming units (CFU). The line represents the optical density of the culture, while bars represent the CFU/ml. The experiment was repeated four times and comparable results were obtained. The figure shows one representative experiment.