Figure 5. Effect of 5k on microtubules and nuclear condensation.

5k induces microtubules depolymerization in HT-29 cells. HT-29 cells were treated with CA-4 (20 nM) or 5k (32.5 nM) for 12 h. After incubation, cells were fixed, reacted with monoclonal anti-tubulin antibody at 4 °C overnight and then reacted with fluorescein isothiocyanate (FITC) conjugated secondary antibody, DAPI was used for nuclear counter-staining, the cellular microtubules were observed under fluorescence microscope (scale bar = 20 μm).