Skip to main content
. 2017 Feb 27;7:43466. doi: 10.1038/srep43466

Figure 4. Cd impairs lysosomal function in cultured Neuro-2a cells.

Figure 4

(a) Representative western blot and quantification analysis for LAMP1 protein in Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, or 24 h). ACTB was used as an internal standard for protein loading. (b) DQ-BSA cleavage activity of Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, or 24 h). (c) CTSD activity of Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, or 24 h). (d) Lysosensor DND-189 fluorescence intensity of Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, or 24 h). The results are expressed as fold changes compared with the control. The values are presented as the means ± SEM. *p < 0.05, **p < 0.01 versus the control group. (n = 4).