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. 2017 Feb 27;7:43466. doi: 10.1038/srep43466

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Copyright © 2017, The Author(s)

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(a) Representative western blot and quantification analysis for LAMP1 protein in Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, or 24 h). ACTB was used as an internal standard for protein loading. (b) qRT-PCR analysis revealing fold expression of TFEB in Neuro-2a cells treated with 50 μM Cd for different times (0, 6, 12, 24 h). (c) Immunofluorescence of Neuro-2a cells incubated with anti-TFEB antibody and DAPI after Cd (50 μM) treatment for 24 h; scale bar: 20 μm. (d) Relative mRNA levels of TFEB target genes were determined after Neuro-2a cells were treated with 50 μM Cd for 24 h. The results are expressed as fold changes compared with the control. The values are presented as the means ± SEM. **p < 0.01 versus the control group. (n = 6).