Table 1.
Primers used for Real-time PCR
| Gene | Primer sequences (5′to3′) | Accession no. |
|---|---|---|
| actin beta | f-TTGTTGACAATGGCTCCGGT r-TCTGGGCTTCATCACCAACG |
NM_205518.1 |
| TLR4 | f-TGAAAGAGCTGGTGGAACCC r-CCAGGACCGAGCAATGTCAA |
NM_001030693.1 |
| MyD88 | f-AGGATGGTGGTCGTCATTTC r-TTGGTGCAAGGATTGGTGTA |
NM_001030962.2 |
| NF-κB | f-CTACTGATTGCTGCTGGAGTTG r-CTGCTATGTGAAGAGGCGTTGT |
M86930.1 |
| TNF-α | f-CAGATGGGAAGGGAATGAAC r-CACACGACAGCCAAGTCAAC |
AY765397.1 |
| IL-1β | f-ACCTACAAGCTAAGTGGGCG r-ATACCTCCACCCCGACAAGG |
NM_204524.1 |
| TGF-β | f-ATGTGTTCCGCTTTAACGTGTC r-GCTGCTTTGCTATATGCTCATC |
NM_205454.1 |
| caspase-3 | f- TCCACCGAGATACCGGACTG r- ACAAAACTGCTTCGCTTGCT |
NM_204725.1 |
| caspase-8 | f- CGGATCAATCGAATAGACCTTC r- CGGCATTGTAGTTTCAGGACTT |
NM_204592.2 |
| PCNA | f- TCTGAGGGCTTCGACACCTA r- AACCTTTTCCTGATTTGGTGCTT |
NM_204170.2 |