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. 2017 Feb 27;10:53. doi: 10.1186/s13068-017-0738-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Engineering the ER to Golgi vesicle trafficking improved the display efficiency of heterologous proteins. a The cell activity of CelA in the ER to Golgi vesicle trafficking engineered strains. b The cell activity of BGL1 in the ER to the Golgi vesicle trafficking engineered strains. c FACS analysis of CelA display efficiency in the ER to Golgi vesicle trafficking engineered strains. Control: the strains without expressing heterologous protein was used as the negative control. The black bars represent 36 h, the gray bars represent 72 h. The data are presented as the means ± standard errors from two independent experiments. *p value of the marked sample vs. control (PYX) <0.05