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Primers for qPCR
| Genes | Number (GenBank) | Sequence (5’-3’) |
|---|---|---|
| MuRF-1 | NM_080903.1 | S: TGACCAAGGAAAACAGCCAC CAG |
| A: TCACT CCTTCTTCTCGTCCAGGATGG | ||
| Atrogin-1 | NM_133521.1 | S: TACTAAGGAGCGCCATGGATACT |
| A: GTTGAATCTTCTGGAATCCAGGAT | ||
| Myostatin | NM_019151.1 | S: CTACCACGGAAACAATCATTACCA |
| A: AGCAAC ATTTGGGCTTTCCAT | ||
| GAPDH | NM_001034034 | S: AGATGGTGAAGGTCGGAGTG |
| A: GAAGGTCAATGAAGGGGTCA |
S – sense; A – anti-sense
