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. 2017 Jan 15;7(3):664–676. doi: 10.7150/thno.15162

Figure 7.

Figure 7

miR-17-3p Protects Against Myocardial Ischemia-Reperfusion Injury. (A) miR-17-3p agomiR significantly increased miR-17-3p in hearts from both sham control and IRI mice. n=6 per group. (B) miR-17-3p agomiR preserved fractional shortening (FS) and ejection fraction (EF) in IRI mice (n=6-7 for control and n=10-11 for IRI mice per group). (C and D) miR-17-3p agomiR attenuated cardiac apoptosis as determined by TUNEL staining (C) and the ratio of Bcl-2/Bax and cleaved caspase 3 (D). At least 4000 cells were quantified in each group. n=3 per group for Western blot. (E) miR-17-3p agomiR attenuated cardiac fibrosis as evidenced by Masson's Trichrome staining (n=3 per group) and expression levels of α-SMA, Collagen I, and Collagen III (n=6 per group). (F) miR-17-3p agomiR further increased the marker of cardiomyocyte proliferation (Ki-67) in IRI mice as determined by immunohistochemical staining. n=3 per group. Data are shown as mean±SEM. Scale bar: 100 μm. *, P<0.05, **, P<0.01, ***, P<0.001 versus respective control.